Hyaluronic Acid Hydrogel Cell Culture

Cell culture and encapsulation.

Hyaluronic acid hydrogel cell culture. A hydrogel was formed within 5 min after mixing with pipette for five times. Hyaluronic acid ˌ h aɪ. Hyaluronic acid hydrogel integrated with mesenchymal stem cell secretome to treat endometrial injury in a rat model of asherman s syndrome feiran liu department of gynecology and obstetrics beijing hospital national center of gerontology beijing 100730 china. Of the synthetic and naturally derived biomaterials that have been examined hyaluronic acid ha based hydrogels have attracted much interest.

Ha is the simplest glycosaminoglycan a negatively charged linear non sulfated polysaccharide that provides compression strength lubrication and hydration within the ecm 1. Conjugate base hyaluronate also called hyaluronan is an anionic nonsulfated glycosaminoglycan distributed widely throughout connective epithelial and neural tissues it is unique among glycosaminoglycans in that it is nonsulfated forms in the plasma membrane instead of the golgi apparatus and can be very large. Their main component is one of the major constituents of the extracellular matrix ecm hyaluronic acid ha. ə l j ʊəˈr ɒ n ɪ k.

These results are useful for designing conditions for using gelatin hyaluronic acid composite hydrogels. Here we report that culture of human ns pcs in 3d hyaluronic acid ha rich biomaterial microenvironments increased differentiation toward oligodendrocytes and neurons over 2d cultures on laminin coated glass. The ratio of hydrazine to aldehyde was 1 1 in all samples with different final ha concentrations. Hystem is a semi synthetic 3d hydrogel platform that allows cell growth in a 3d environment.

Stem cell marker genes nanog oct4 and sox2 were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes. This work reports a thermoresponsive hyaluronic acid and poly n isopropylacrylamide based hydrogel that supports human pluripotent stem cell culture from single cells. The material can be chemically and mechanically tuned and provides a fully defined and scalable method for stem cell expansion.