Human Lung Fibroblast Cell Line
Lung disease.
Human lung fibroblast cell line. 2 continuous lung cell lines. Normal human atcc pcs 201 013 from the liquid nitrogen tank. Human fetal lung fibroblasts such as hfll mrc 5 and wi38 have been extensively studied and characterized in aging studies collagen biosynthesis and cytotoxicity testing. Human cell type.
Jacobs the cell line was derived from the human lung tissue of a 14 week old male fetus aborted from a 27 year old woman. The human lung fibroblast cell line mrc 5 constitutively produces a megakaryocyte potentiator activity identified in murine bone marrow liquid culture assays for acetylcholinesterase and megakaryocyte colony assays in the presence of low concentrations of il 3. The cells are derived from a first trimester fetus. Bladder cardiac dermal gingival lung airway prostate scleral uterine and vas deferens.
Cc 2512 and fgm tm 2 bulletkit tm growth media catalog no. Initiated in september 1966 by j. Make sure that the caps of all components are tight thaw the components of fibroblast growth kit low serum atcc pcs 201 041 just prior to adding them to the fibroblast basal medium atcc pcs 201 030. Ag06814 j fibroblast from skin lung ag05965 c fibroblast from skin lung search results for aborted fetus.
Jacobs in september of 1966. The modal chromosome number was 46 occurring in 70 of cells. Purchase our lung fibroblast cell culture kit catalog no. Wi 38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3 month gestation aborted female fetus.
The kit consists of a cryopreserved ampule of lung fibroblast cells catalog no. The production levels of this activity were increased after stimulation with the. Complete growth medium obtain one vial of primary lung fibroblast cells. Cell collections a publication of the coriell cell repositories 2003 2004.
26 29 the fibroblasts are easily maintained in culture and exhibit a finite life span. Lifeline normal human fibroblasts provide an ideal cell system to study wound healing toxicology cancer or basic cell biology in various organs including skin lung bladder and the reproductive systems. A certificate of analysis is provided for each cell lot purchased. The cell line isolated by leonard hayflick in the 1960s has been used extensively in scientific research with applications ranging from developing important theories in molecular biology and aging to the production of most human virus vaccines.
Our normal human fibroblasts can also be used for drug screening. Fluorescence digital image gallery human fetal lung fibroblast cells mrc 5 line the mrc 5 cell line is commonly utilized in vaccine development as a transfection host in virology research and for in vitro cytotoxicity testing. Fibroblast tissue. A brief history of imr 90.