Human Bronchial Epithelial Cell Line

Count the cells with a hemocytometer or cell counter.

Human bronchial epithelial cell line. Primary human bronchial epithelial cells hbepc are isolated from the surface epithelium of human bronchi and stain positive for cytokeratin. Aspirate the supernatant from the tube without disturbing the cell pellet. 16hbe14o human bronchial epithelial cell line 16hbe14o human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of cftr. 16hbe14o is a human bronchial epithelial cell line isolated from a 1 year old male heart lung patient and immortalized with the origin of replication defective sv40 plasmid psvori.

Resuspend the cells in 2 ml of bronchial tracheal epithelial growth medium by gently pipetting the cells to break up the clumps. These cells are ideally suited to support the study of many fields including inflammation cancer gene regulation cell matrix interactions and also have application in toxicology testing and drug screening development. Flick the tip of the conical tube with your finger to loosen the cell pellet. Atcc normal human primary bronchial tracheal epithelial cells when grown in airway epithelial cell basal media supplemented with bronchial tracheal epithelial cell growth kit components provide an ideal cell system to propagate bronchial tracheal epithelial cells in serum free conditions.

Further observation indicates distinct viral replication with a dynamic. Find msds or sds a coa data sheets and more information. 16hbe14o cells have a cobblestone appearance in culture form tight junctions and display. The 16hbe14o cell line was originally developed from human bronchial epithelium to study the cystic fibrosis transmembrane conductance cftr regulator cozens et al 1994 and retains many features of differentiated bronchial epithelial cells.

The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. Calu 3 cells human bronchial epithelial cell line are originally derived from the subbronchial epithelium of a lung adenocarcinoma atcc manassas va and have been used as surrogate nasal epithelium due to their similar biophysical properties such as forming tight monolayers and also secreting mucus when cultured in an air liquid. Investigation of this viral infection in the human bronchial epithelial cell line 16hbe suggests that sars cov 2 can enter these cells through interaction between its membrane localized s protein with the angiotensin converting enzyme 2 molecule on the host cell membrane.